Three Purkinje cells from guinea-pig cerebellar slices were physiologically
characterized using intracellular techniques. The neurons were fully
reconstructed, following physiological measurements and staining with
horseradish peroxidase. Reconstructions of the neurons were made using
'Neuron tracing system' (Eutectic Inc.). Using this combined software and
hardware system, the neuron is represented by a set of points where each
point has the following attributes: type, x, y, z, coordinates and a
diameter. The Purkinje cells images are built of 1400-2100 points and give
an accurate description of the neurons morphology. Note, however, that
dendritic spines are not included in the morphological description; they
were added globally into the model utilizing the method of Rapp et al.
(1992).
The morphological description of these 3 Purkinje cells (in Eutectic
format) is now available and can be fetched from the following WWW site:
http://www.ls.huji.ac.il/~rapp
For physiological and modeling details see:
Rapp M., Yarom Y. and Segev I. (1992) "The impact of parallel fibers
background activity on the cable properties of cerebellar Purkinje cells".
Neural Computation 4, 518-533
Rapp, M., Segev, I. and Yarom. Y. (1994) "Physiology, morphology and
detailed passive models of guinea-pig cerebellar Purkinje cells".
Journal of Physiology 474.1, 101-118