Vitamin E Colorimetric ELISA Assay Kit

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. 

VE content can be calculated by measuring the OD value at 532 nm.

Synonyms VE

Sample Type Serum,Plasma,Tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (533 nm)
Research Area Oxidative Stress,  Plant Physiology Series
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Absolute ethanol, N-heptane
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.09 μg/mL
Detection Range 0.09-40 μg/mL
Precision inter-assay CV: 4.9% | intra-assay CV: 4.4%
Sample Volume 0.3 mL
Assay Time 1 h